Topspec – Next generation precision antibody profiling – from science fiction to reality

The covid19 pandemic

TopSpec was initially planned to run from Jan 2019 – Dec 2021. The first reporting period (Jan 2019-Dec 2019) had no major deviations, however, due to the corona outbreak in February 2020, two 6 months extensions of the project were amended.

Updated Gantt chart:

Gantt

Deliverables:

D9.1: Logo and Website launch and public accessibility. – Approved. D9.1

D8.1: IP protection strategy finalized.  – Approved.

D9.2: Data management plan. – Approved. D9.2

New version: D9.2_v2

D6.1: Modification of the Orbitrap mass spectrometer. – Approved.

D8.2: Exploitation and Dissemination Plan. – Approved. D8.2

Newest version: D8.2_v3

D9.3: Technical/scientific review meeting documents – Approved

D3.1: Prototype of the HAB gun installed and tested protocol –
Submitted 31/3 – 2020 – Approved

D2.1: In situ testing of the optimized CAD MS/MS protocol –
Submitted 30/6 – 2020 – Approved

D2.2: Protocol of in situ testing of the optimized CAD –
Submitted 28/8 – 2020 – Approved

D5.1: Prototype pI-Trap-ESI installed and tested– protocol –
Submitted 31/8 – 2020 – D5.1 – Approved

D2.3: In situ testing optimized ECD, HECD and EID MS/MS –
Submitted 29/6 – 2021 – Approved

D3.2: Optimized HAB guns installed and tested – protocols –
Submitted 29/6 – 2021 – Approved

D4.1: Protocol- CED gun prototype installed and tested –
Submitted 29/6 – 2021 –  Approved

D9.4: Scientific review meeting documents –

Submitted 23/7 – 2021 –  Approved

D7.1: Two Prototype FTMS Booster installed and tested-protocol –
Submitted –  1/10 – 2021

D7.2: Top-down analysis software –

Submitted – 30/11 – 2021 –

D4.2: Protocols: CED guns installed and tested –

Submitted – 30/3 -2022

D1.2: Modified Omnitraps with updated software

Submitted – 30/3 -2022 – D1.2

D8.3: Young scientist TopSpec technology workshop –

Submitted – 30/3 -2022 D8.3

D1.1:  Two fully equipped Omnitraps & one IMS installed –

Submitted – 31/10 -2022 D1.1

D1.3: Fully serviced, functioning Omnitraps & IMS –

Submitted – 16/11 -2022 D1.3

D6.2: Modified Orbitrap Q Exactive HF X installed –

Submitted – 30/11 -2022 D6.2

D7.3: Optimized FTMS Boosters test protocols –

Submitted – 12/12 -2022

D8.4: Public demonstrations of TopSpec technology

Submitted – 22/12 -2022 D8.4

D8.5: Scientific reports and publications

Submitted – 27/12 -2022 D8.5

New version: 13/1 – 2023 D8.5_v2

D5.2: Two tested, optimized pI-Trap-ESI installed and tested

Submitted – 28/12 -2022 D5.2

D8.6: Exploitation plan and Business strategy document

Submitted – 31/12 -2022

D9.5: Final: Technical/scientific review meeting documents

Submitted – 19/1 – 2023

Milestones:

1. Demonstrated effectiveness of product ion isotopic distribution deconvolution. – Achieved.

2. Omnitraps & IMS Electronics design. – Achieved.

3. Installation of Q Exactive instrument for Omnitrap development. – Achieved.

4. Omnitraps & IMS P.O.s sent to suppliers. – Achieved.

5. Omnitraps & IMS Mechanical design.  – Achieved.

6. Data processing algorithms and software for simulated and experimental top-down mass spectra and time-domain data transients – Achieved.

7. Suffic HAB MS/MS demonstrated – Achieved.

8. Interfacing pI-Trap-Orbitrap. – Achieved.

9. Interfacing pI-Trap-Orbitrap OMNI-ORBI combination. – Achieved.

10. Data analysis algorithms and software for simulated and experimental top-down data analysis. – Achieved.

M11. Two FTMS Booster prototypes are designed, implemented, and evaluated – Achieved.

M12. Development of CED MS/MS – Achieved.

M13. All technologies interfaced – Achieved.