Topspec – Next generation precision antibody profiling – from science fiction to reality

Patents:

Submitted:

• Biomotif: Tandem high resolution pI Trap,Swedish Patent application no. 2030184-2
• Fasmatech: Hybrid hyperthermal Hydrogen atom-ion source, submitted at EPO and UKPTO

Articles:

Interlaboratory Study for Characterizing Monoclonal Antibodies by Top-Down and Middle-Down Mass Spectrometry, Yury O. Tsybin et al, J. Am. Soc. Mass Spectrom. 2020, 31, 9, 1783–1802; https://doi.org/10.1021/jasms.0c00036

Transient-Mediated Simulations of FTMS Isotopic Distributions and Mass Spectra to Guide Experiment Design and Data Ana lysis, Yury O. Tsybin et al, J. Am. Soc. Mass Spectrom. 2020, 31, 9, 1927–1942; https://doi.org/10.1021/jasms.0c00190

Exploring frontiers of orbitrap performance for long transients, Alexander Makarov et al, International Journal of Mass Spectrometry 466 (2021) 116607; https://doi.org/10.1016/j.ijms.2021.116607

Software:

Improved assignment algorithms for antibody spectra that will be used for TopSpec:

C. Gstöttner, D. Reusch, M. Haberger, I. Dragan, P. van Veelen, D.P.A. Kilgour, Y.O. Tsybin, Y.E.M. van der Burgt, M. Wuhrer & S. Nicolardi (2019): Monitoring glycation levels of a bispecific monoclonal antibody at subunit level by ultrahigh resolution MALDI FT-ICR mass spectrometry